Osmolarity,cell volume and proliferation in nasopharyngeal carcinoma cells

AIM: To investigate the relationship between osmolarity, cell volume and cell proliferation in nasopharyngeal carcinoma cells. METHODS: MTT method was applied to detect the proliferation ability of the poorly-differentiated nasopharyngeal carcinoma cell (CNE-2Z) under various osmolarity conditions. The flow cytometry was used to analyse cell cycle distribution. Cell volume was obtained by the image analysis of living cells and cell viability was determined by the trypan blue assay. RESULTS: Cultivation of cells under the hypertonic conditions of 370 and 440 mOsmol/L increased cell volume by 8.7% and 27.8% and facilitated cell proliferation by 22.2% and 33.9%, respectively. However, hypotonic incubation of cells with osmolarity of 160 and 230 mOsmol/L decreased cell volume by 12.8% and 4.1% and inhibited cell proliferation by 34.0% and 15.6%, respectively. Cell volume was positively correlated with cell proliferation rate. Long-term cultivation of cells under anisotonic conditions did not significantly alter cell cycle distribution, but hypotonic cultivation decreased cell viability. CONCLUSION: Proliferation of nasopharyngeal carcinoma cells was closely correlated with the osmolarity of culture medium and cell volume. Hypotonic cultivation may inhibit cell proliferation by decreasing cell volume to facilitate cell death mechanisms.     

Effect of indomethacin on tumor invasion in human laryngeal cancer Hep-2 cells

AIM: To assess the effect of indomethacin on tumor invasion in a human laryngeal cancer Hep-2 cell line in vitro. METHODS: Hep-2 cells were exposed to indomethacin at different concentrations for 48 h. Then cell growth rate, the colony formation in soft agar medium and cell mobility were examined, and monolayer invasion assay was performed to assess cell invasion index. RESULTS: Preteatment with indomethacin inhibited the colony formation of Hep-2 cells and the cell mobility, and decreased the invasion index. CONCLUSION: Indomethacin can inhibit the invasion of Hep-2 cells.     

Role of vascular NAD(P)H oxidase in vascular remodeling

NAD(P)H oxidase was initially found in phagocytes and it participates in the generation of reactive oxygen species(ROS). Recent researches have showed that NAD(P)H oxidase also expresses in other tissues including blood vessels and it plays a critical role in vascular remodeling through ROS which are important signaling molecules in vascular cells.This article reviews the biochemical characterization, activation paradigms, structure, and function of this enzyme.     

丝状霉形体丝状亚种SC型脂蛋白LppQ N末端基因克隆与序列分析

脂蛋白LPPQ是丝状霉形体丝状亚种SC型(MmmSC)非洲株、欧洲株和疫苗株所特有的。LPPQ N末端域具有良好的免疫原性，在牛体内可诱导产生强大、特异、早期、持续的免疫反应。本研究根据已发表的LPPQ基因序列设计引物，用Pyrobest^TM高保真DNA聚合酶从MmmSC HVRI X株中扩增出了LPPQ N末端基因序列，并进行了克隆与序列测定。核苷酸序列比较结果显示，HVRI X株的LPPQ N末端基因序列与国外发表的序列同源性为99．7％，由其推导的氨基酸序列同源性为99，1％，为脂蛋白LPPQ N末端基因体外表达奠定了基础。     

二丁酰环腺苷酸的合成与性质研究

以ATP为原料采用化学方法合成了dbcAMP及其钠盐 ,并对其理化性质和吸收特性进行了研究。经质谱分析 ,其结构为C18H2 3 N5O8PNa ,分子量为 4 90 .0 ;经紫外光谱测定其含量为 96 .0 % ,产品的得率为 85 .6 %。dbcAMP钠盐为白色粉末 ,可溶于水与乙醇 ,对热和酸碱比较稳定。经饲料添加和皮下注射dbcAMP检测其经消化道和皮下吸收的性能。通过测定饲喂和皮下注射dbcAMP后 0 .5h、1～ 96h不同时间内血液dbcAMP的浓度表明 ,饲料添加在 3h内达到最大浓度 84 .75pmol/mL ,皮下注射在 2h内达到最大浓度 92 .96pmol/mL ,之后血液中dbcAMP的浓度逐渐下降 ,在 96h达到较低水平。     

二丁酰环腺苷酸对猪胴体组成和肉品质的影响

通过皮下注射和饲料添加不同剂量dbcAMP进行饲养试验 ,研究其对肥育猪胴体组成和肉品质的影响。结果表明 :皮下注射 1.0mg/kg和饲料添加 2 0mg/kg对改善肥育猪的胴体组成和肉品质效果最好 ,眼肌面积分别提高31.99%和 36 .39% (P <0 .0 5 ) ;瘦肉率分别提高 12 .4 2 %和 11.94 % (P <0 .0 5 ) ;背膘厚分别降低 19.95 %和 2 0 .97%(P <0 .0 5 )。肌肉粗蛋白含量分别提高 10 .87%和 16 .5 4 % (P <0 .0 5 )。     

洛南县秦川牛改良山地黄牛调查报告

秦川牛是我国著名地方良种黄牛 ,我县从 2 0世纪 5 0年代开始引进秦川种公牛改良当地黄牛 ,2 0世纪 70年代末推广牛的冷配技术 ,引进秦川种公牛冷冻精液颗粒、细管 ,坚持本交与人工授精相结合。全县累计引进秦川牛 670 0多头 ,改良配种 9.0 1万头 ,产改良牛 6.5 8万头。秦川牛的引进 ,丰富了我县黄牛遗传基因库 ,优化了牛群整体结构 ,改良效果明显 ,社会经济效益显著     

大熊猫犬冠状病毒的分离与鉴定

以犬肾传代细胞(MDCK)从1只病死大熊猫肝脏中分离到1株病毒,命名为DXMV。电镜观察磷钨酸负染的病毒细胞培养物，发现冠状病毒样粒子，超薄切片上可见细胞浆内病毒包涵体。理化学和生物学鉴定结果表明，DXMV核酸类型为RNA，对氯仿、乙醚敏感，可耐受1％胰蛋白酶的作用，具有一定的耐酸性和耐热性，可在MDCK、FCWF、F81细胞中增殖，无血凝性和血吸附特性。血清中和试验结果表明，DXMV可被犬冠状病毒阳性血清中和。采用犬冠状病毒间接荧光抗体法染色DXMV细胞飞片，可在细胞浆内见到特异性荧光。以冠状病毒通用引物和犬冠状病毒特异性引物，可从大熊猫肝脏和不同代次的病毒细胞培养物中扩增出与预期值251bp和515bp大小相同的核苷酸片段。由此说明，该病毒为犬冠状病毒，大熊猫可被犬冠状病毒感染。     

松嫩平原优势种羊草与其主要伴生种芦苇空间分布格局分析

芦苇是松嫩草地优势种羊草的主要伴生种,两者常常形成物种组成比较单一的混生群落或者与其他物种组成羊草 杂类草群落.选择羊草 芦苇混生群落,羊草群落和芦苇群落的交错区进行羊草、芦苇单物种格局分析.结果表明,1)混生群落和交错区中,羊草和芦苇均是以集聚分布的形式存在;2)羊草在混生群落中的格局强度小于交错区中的格局强度,芦苇在2种类型群落中的格局强度没有显著差异;3)除了考虑物种的生物学特性以外,混生群落种内、种间竞争也是影响羊草集聚分布和格局强度的主要因素,交错区中,环境异质性和种间竞争是影响集聚分布和格局强度的重要因子;4)芦苇在混生群落和交错区中格局强度没有明显差异,由此可以判定芦苇的空间分布格局形成主要受植物本身生物学特性的影响.     

狗牙根辐射诱变后代外部性状变异分析

对1997-2002年经过辐射诱变得到的狗牙根诱变后代的6个坪用性状指标的统计分析结果表明,1)不同的辐射诱变剂量处理的后代间坪用性状有显著差异,尤其在节间直径和密度上,6 800 rads处理的后代节间都比9 000 rads处理的后代粗,而密度是前者低于后者,2种剂量处理都可以显著降低草层高度;2)不同辐射诱变代数的后代间存在着差异,原始种源进行多代的辐射诱变,可以使得叶片显著变窄,草层高度显著降低,节间变细变长; 3)同一材料在相同辐射剂量诱变处理后匍匐茎不同节之间也存在显著的差异.